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Ena Orzech


Ena Orzech

Merck Life Sciences R&D, Israel

Biography

Ena Orzech is working at Merck Life Sciences R&D and has 20 years’ experience in new product development, specializing in protein expression and purification. During her work she has development variety of recombinant proteins and had designed different kits. During the present work she gained extensive experience in protein expression in E.coli bacteria expression system and in Pichia pastoris. Expression in Pichia pastoris system includes cloning into Pichia expression vectors, transformation, screening for stable clones, expression and purification.

Abstract

Abstract : A novel Mucin-selective protease StcE is a powerful tool for heavily O-glycosylated mucin proteins MS analysis